Higher education is facing increased enrollments, requests to expand its online course offerings, and a need to find alternative ways to deliver laboratories for science courses due to high demand for them by both major and non-major students. At the same time, the high cost of expanding these laboratory facilities are creating bottlenecks. The North American Network of Science Labs Online (NANSLO), established in 2012, has three web-based science laboratories. Using a web interface, students access an assigned lab activity and, using real high-end scientific equipment, generate real data, collaborate with other students, and collect information used to complete their labs all done in real time. NANSLO currently has 27 lab activities available in biology, chemistry, allied health, and physics that can be used as is or customized easily to meet course needs. With over 2,000 students served to date, NANSLO is capable of delivering high quality NANSLO laboratory experiences to students via the Internet anywhere they are located at a time convenient for them.