Higher education institutions are facing growing pressure to expand online course offerings. This has led to a need for alternative ways to provide laboratory learning experiences for science courses due to high demand for them by both major and non-major students. At the same time, the high cost of expanding existing or building new laboratory facilities is making this prohibitive, resulting in enrollment bottlenecks. The North American Network of Science Labs Online (NANSLO), established in 2012, includes two web-based science laboratories available for use by institutions on a fee-for-service model. Using a web interface, students access an assigned lab activity and, using real high-end scientific equipment, generate real data, collaborate with other students, and collect information used to complete their labs−all done in real time. NANSLO currently has 28 lab activities available in biology, chemistry, allied health, and physics, and two lab manuals−NANSLO Survey of Chemistry and NANSLO Survey of Biology Lab Manuals, each containing 10 lab activities−used in non-science and science major courses. All of these can be used as-is or customized by faculty to meet course needs. With over 2,000 students served to date, NANSLO is capable of delivering high-quality NANSLO laboratory experiences to students via the Internet wherever they are located at a time convenient for them.